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Sample-Handling Friendly Slanted Top Quartz Cuvette with Black Walls for Spectroscopy -TOQUARTZ®
Features of Slanted Top Quartz Cuvette with Black Walls
TOQUARTZ® Slanted Top Quartz Cuvettes with Black Walls are engineered for precision spectroscopy applications where minimal light scatter and easy sample handling are critical. These cuvettes combine high-purity quartz material with specialized design features to deliver optimal performance in both routine and advanced analytical procedures.
Black Wall Design
Specialized black walls prevent light scattering and cross-talk, reducing background interference in fluorescence applications and improving signal-to-noise ratio in sensitive measurements.
Slanted Top Opening
The ergonomic slanted opening design facilitates easy sample loading and minimizes contamination risk, especially valuable when working with precious micro-volume samples.
High-Purity Quartz Material
Manufactured from ≥99.98% SiO₂ quartz, ensuring excellent chemical stability and optical clarity throughout the UV-Vis-NIR spectrum (200-2500nm) with no absorption peaks.
- Optical transmission: 200nm-2500nm
- Thermal stability: Up to 100°C
- Customization available
Technical Specifications & Dimensions of Slanted Top Quartz Cuvette with Black Walls
Technical Specifications
Physical Properties
| Parameter | Specification |
| Material | High-purity quartz (≥99.98% SiO2) |
| Surface Finish | Polished optical windows |
| Black Wall Coating | Chemically stable, non-fluorescent |
| Thermal Expansion | 5.5×10-7/℃ |
| Density | 2.2 g/cm3 |
Chemical Properties
| Parameter | Specification |
| Chemical Resistance | Stable against most acids, bases, and organic solvents |
| pH Stability Range | 1-14 |
| Solvent Compatibility | Compatible with aqueous and organic solvents |
| Surface Adsorption | Minimal protein/nucleic acid binding |
| Hydrophobicity | Moderate (contact angle ~30°) |
Optical Properties
| Parameter | Specification |
| Transmission Range | 200-2500nm |
| Transmission Efficiency | >80% (UV-Vis range) |
| Refractive Index | 1.46 at 589nm |
| Fluorescence | Negligible intrinsic fluorescence |
| Black Wall Light Absorption | >99% |
Size Chart
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4003-1 | 350μl Slanted Top Quartz Cuvette with Black Walls | 200nm-2500nm | 10mm | 350μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4003 | 700μl Slanted Top Quartz Cuvette with Black Walls | 350nm-2000nm | 10mm | 700μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4003-4 | 1050μl Slanted Top Quartz Cuvette with Black Walls | 200nm-2500nm | 10mm | 1050μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4003-2 | 1400μl Slanted Top Quartz Cuvette with Black Walls | 200nm-2500nm | 10mm | 1400μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4003-3 | 1750μl Slanted Top Quartz Cuvette with Black Walls | 200nm-2500nm | 10mm | 1750μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4033-1 | 2100μl Slanted Top Quartz Cuvette with Black Walls | 200nm-2500nm | 30mm | 2100μl | 100℃ | 12.5*32.5*45mm |
TOQUARTZ® Solving Analytical Challenges with
Slanted Top Quartz Cuvettes
Slanted Top Quartz Cuvette with Black Walls in DNA Quantification for NGS Sample Prep
Key Advantages
-
Reduced pipetting loss to <2%
Slanted top geometry enables precise pipette tip placement, minimizing residual volume during DNA transfer. -
Stray light suppression improves OD260 accuracy by 18%
Black walls eliminate side scatter, enhancing UV absorbance signal clarity in low-concentration DNA samples. -
Supports accurate quantification at 2 ng/μl
Optimized optical path and low background noise allow reliable detection of sub-microgram DNA concentrations.
TOQUARTZ® solution
A genomics lab in Germany reported inconsistent DNA quantification using standard cuvettes, with OD260/280 ratios fluctuating ±0.15 and 12% of samples failing NGS library QC. After switching to TOQUARTZ Slanted Top Quartz Cuvettes with Black Walls, pipetting loss dropped below 2%, and quantification variance reduced to ±0.03, enabling >98% library pass rate.
Slanted Top Quartz Cuvette with Black Walls in Fluorescent Protein Assays in Biotech R&D
Key Advantages
-
Background fluorescence reduced by 92%
Black wall coating eliminates wall-induced autofluorescence, critical for low-intensity GFP/RFP assays. -
±0.05mm alignment tolerance across batches
Slanted top ensures consistent sample positioning, reducing inter-sample variability in fluorescence readings. -
Improves replicate consistency by 4×
Enhanced optical isolation and sample geometry yield CVs <2% across 96-well equivalent workflows.
TOQUARTZ® solution
A US-based biotech startup developing GFP-tagged antibodies faced 18–22% coefficient of variation (CV) in fluorescence intensity across replicates using standard cuvettes. After adopting TOQUARTZ Slanted Top Quartz Cuvettes with Black Walls, CV dropped to 4.8%, enabling reliable quantification of expression levels and batch-to-batch QC in preclinical studies.
Slanted Top Quartz Cuvette with Black Walls in Enzyme Kinetics Using UV-Vis Spectroscopy
Key Advantages
-
Reagent mixing time reduced by 40%
Slanted opening allows direct reagent injection into the light path, accelerating reaction initiation. -
Bubble formation rate <3%
Angled geometry minimizes turbulence and air entrapment during rapid pipetting in kinetic assays. -
Initial rate (V₀) deviation reduced to ±0.02 AU/min
Black walls stabilize baseline absorbance, improving accuracy of early-phase kinetic measurements.
TOQUARTZ® solution
A pharmaceutical R&D team in Japan observed erratic V₀ readings in enzyme inhibition assays due to delayed mixing and bubble interference, with baseline drift up to ±0.08 AU. After switching to TOQUARTZ Slanted Top Quartz Cuvettes with Black Walls, mixing time dropped by 40%, and baseline deviation stabilized at ±0.01 AU, improving kinetic curve fitting and reducing assay repeat rate by 35%.
TOQUARTZ® Customization Options for Slanted Top Quartz Cuvette with Black Walls
Dimensional Modifications
- Custom path lengths
- Modified internal volumes
- Altered external dimensions for specialized instruments
- Optimized slant angles for specific pipetting systems
- Extended height options for greater sample volumes
Surface & Coating Options
- Partially blackened walls for specialized applications
- Selective window areas for multi-angle fluorescence
- Anti-reflective coatings for specific wavelengths
- Hydrophobic surface treatments for improved filling
- Frosted areas for identification and handling
Special Features
- Integration of mixing elements for kinetic studies
- Custom stoppers or lids for volatile samples
- Temperature control channels for thermal studies
- Flow-through modifications for continuous measurement
- Compatibility features for automated systems
Usage Guide of Slanted Top Quartz Cuvette with Black Walls
Proper Handling & Sample Loading
- Hold cuvettes by the frosted or opaque sides only, never touch optical windows with bare fingers
- When filling, position pipette tip against the slanted wall and dispense slowly to prevent bubble formation
- Fill to appropriate volume - ensure the meniscus is below the light path for accurate measurements
- Inspect for bubbles by holding against light source; if present, gently tap side of cuvette to release
- Insert cuvette into spectrophotometer with clear optical windows facing the light path
Cleaning & Maintenance
- Rinse immediately after use with appropriate solvent (typically deionized water or methanol)
- For thorough cleaning, soak in 2% Hellmanex™ or similar laboratory detergent for 30 minutes
- Rinse thoroughly with deionized water (at least 5-10 times)
- For stubborn residues, short exposure to dilute nitric acid (0.1M) followed by thorough rinsing is effective
- Air dry in dust-free environment or dry with compressed air/nitrogen
- Store in original packaging or dedicated container to prevent scratches and contamination
Usage Precautions
Do's:
- Equilibrate samples and cuvettes to the same temperature before measurement
- Use lint-free optical tissues for cleaning optical surfaces
- Perform baseline correction with identical buffer/solvent
- Standardize handling protocols for reproducible results
- Check optical windows for cleanliness before each measurement
Don'ts:
- Avoid ultrasonication as it may damage black wall coating
- Never use abrasive materials or brushes on optical surfaces
- Don't expose to temperatures above 100°C
- Avoid prolonged contact with strong alkaline solutions (>10M NaOH)
- Don't use concentrated HF (hydrofluoric acid) which attacks quartz
Ready to Enhance Your Spectroscopic Analysis?
Why Partner with TOQUARTZ
Direct Factory Advantage
As a direct manufacturer, we can cut out the numerous intermediate links.
Engineering Expertise
Technical team guides clients from material selection to design optimization, translating specs into deliverables.
Flexible Manufacturing
Handling standard & custom orders via small-batch expertise and prototyping rigor to meet urgent deadlines.
Quality
Assurance
Pre-shipment 3-step validation:
1. dimensional accuracy,
2. material purity ,
3. performance thresholds
Global Supply Chain
Reliable global logistics to industrial hubs (DE/US/JP/KR priority) with trackable milestones.
Releted Products
As a specialized manufacturer with direct factory capabilities, TOQUARTZ provides both standard and custom quartz solutions with engineering support throughout the specification and implementation process.
FAQ
Q: What is the purpose of black walls in quartz cuvettes?
A: Black walls prevent light scattering and minimize background noise, particularly important in fluorescence spectroscopy where signal-to-noise ratio is critical. They also reduce cross-talk between adjacent samples in multi-cell holders.
Q: Why choose a slanted top design over standard openings?
A: The slanted opening facilitates easier sample loading, reduces bubble formation, allows for smaller sample volumes, and minimizes contamination risk during pipetting operations. It’s particularly beneficial for precious or limited samples.
Q: Are these cuvettes compatible with standard spectrophotometers?
A: Yes, TOQUARTZ cuvettes are designed with standard 12.5×12.5mm external dimensions to fit most commercial spectrophotometers from manufacturers like Agilent, Thermo Scientific, Shimadzu, PerkinElmer, and others.
Q: What is the chemical composition of the black wall coating?
A: The black coating is a proprietary non-fluorescent, chemically resistant material permanently bonded to the quartz surface. It maintains stability across a wide pH range and is compatible with most common laboratory solvents.
Q: What temperature range can these cuvettes withstand?
A: The standard models are rated for temperatures up to 100°C. While the quartz material itself can withstand much higher temperatures, the black coating limits the maximum operating temperature. For higher temperature applications, special high-temperature coatings are available by custom order.
Contact our engineering team for technical consultation and pricing. We’ll help you select the optimal specifications for your application requirements.