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Stray Light Suppression Quartz Micro Fluorescence Cuvette with Black Walls and Lid for Fluorescence Spectroscopy -TOQUARTZ®
Features of Quartz Micro Fluorescence Cuvette with Black Walls and Lid
TOQUARTZ® Quartz Micro Fluorescence Cuvettes are engineered for precision spectroscopy applications where sample conservation and signal accuracy are paramount. Each cuvette is manufactured from high-grade quartz to deliver consistent optical performance across a wide wavelength range.
Design Features
- Black walls and lid for maximum fluorescence sensitivity
- Micro volume design (20μl, 100μl, 200μl options)
- Secure-fit lid design to prevent sample evaporation
- Compatible with standard spectrophotometer holders
Optical Performance
- UV-VIS-NIR transmission range (200-2500nm)
- Black walls minimize stray light and cross-talk
- Precision polished optical windows (≤ λ/4 flatness)
Material Composition
- High-purity quartz (≥99.98% SiO₂) for optimal optical clarity
- Low thermal expansion coefficient (5.5×10⁻⁷ /°C)
- High chemical resistance to acids, bases, and solvents
- Precision machining for dimensional accuracy
- Individual inspection for defects and optical clarity
- Rigorous quality control and optical testing
Technical Specifications & Dimensions of Quartz Micro Fluorescence Cuvette with Black Walls and Lid
Technical Specifications
| Parameter | Specification | Notes |
| Physical Properties | ||
| Available Volumes | 20μl, 100μl, 200μl | Custom volumes available on request |
| Standard Pathlength | 10mm (±0.1mm) | Custom pathlengths: 1-50mm available |
| Wall Thickness | 1.25mm (±0.1mm) | Uniform thickness for consistent performance |
| External Dimensions | 12.5×12.5×45mm | Compatible with standard cuvette holders |
| Thermal Expansion Coefficient | 5.5× 10-7/℃ | Excellent dimensional stability |
| Chemical Properties | ||
| Material Composition | ≥9.98% SiO2 | High-purity fused quartz |
| Chemical Resistance | Excellent (acids, bases, solvents) | Exception: HF and hot concentrated phosphoric acid |
| Water Absorption | <0.001% | Negligible effect on measurements |
| Optical Properties | ||
| Transmission Range | 120-4500nm | Covers UV, Visible, and NIR regions |
| Refractive Index | 1.458 at 546nm (20℃) | Low dispersion across spectrum |
| Fluorescence | Negligible | Minimal background signal |
| Optical Window Flatness | ≤ λ/4 | Excellent beam quality maintenance |
| UV Cutoff | ~170nm | Superior to standard glass (cutoff ~350nm) |
Size Charts
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4008 | 20μl Quartz Micro Fluorescence Cuvette with Black Walls and Lid | 200nm-2500nm | 10mm | 20μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4010 | 45μl Quartz Micro Fluorescence Cuvette with Black Walls and Lid | 200nm-2500nm | 3mm | 45μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4017 | 50μl Quartz Micro Fluorescence Cuvette with Black Walls and Lid | 200nm-2500nm | 10mm | 50μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4033 | 50μl Quartz Micro Fluorescence Cuvettes with Black Walls and Lid | 200nm-2500nm | 10mm | 50μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4019 | 100μl Quartz Micro Fluorescence Cuvette with Black Walls and Lid | 200nm-2500nm | 10mm | 100μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length | Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4018 | 200μl Quartz Micro Fluorescence Cuvette with Black Walls and Lid | 200nm-2500nm | 10mm | 200μl | 100℃ | 12.5×12.5x45mm |
TOQUARTZ® Solving Analytical Challenges with Quartz Micro Fluorescence Cuvette with Black Walls and Lid
Quartz Micro Fluorescence Cuvette with Black Walls and Lid in Single-Cell RNA Fluorescence Quantification
Key Advantages
-
Minimized Sample Requirement
Supports accurate fluorescence detection with as little as 20μl RNA extract per assay. -
Evaporation Prevention During Thermal Cycling
Black lid reduces sample loss by over 90% during 30-minute qPCR-linked fluorescence assays. -
Stray Light Suppression for Low-Intensity Signals
Black walls reduce lateral light interference by 96%, improving signal-to-noise ratio in sub-nanogram RNA detection.
TOQUARTZ® solution
A genomics lab at a European university faced 18% signal deviation in single-cell RNA fluorescence due to evaporation and stray light using open-top cuvettes. After switching to TOQUARTZ® cuvettes, deviation dropped to 3.2%, and sample volume was reduced from 100μl to 25μl per test, enabling reliable quantification from single-cell lysates.
Quartz Micro Fluorescence Cuvette with Black Walls and Lid for Fluorescent Nanoparticle Tracking in Drug Delivery Research
Key Advantages
-
Ambient Light Isolation for Low-Concentration Tracers
Black walls block >95% of ambient light, enabling detection of 0.05μg/mL fluorescent nanoparticles. -
Reduced Background Fluorescence
Black-coated interior eliminates wall reflection artifacts, improving baseline stability by 40%. -
Secure Lid for Long-Term Tracking
Black lid maintains optical consistency during 60-minute kinetic uptake assays without evaporation.
TOQUARTZ® solution
A US-based biotech startup tracking FITC-labeled liposomes at 0.1μg/mL reported inconsistent readings due to ambient light and cuvette wall reflection. After adopting TOQUARTZ® black wall cuvettes, signal variability decreased by 38%, and background fluorescence was reduced by 42%, enabling reproducible tracking over 1-hour intervals.
Quartz Micro Fluorescence Cuvette with Black Walls and Lid in Enzyme Kinetics Using Fluorescent Substrates
Key Advantages
-
Stable Signal in Time-Resolved Assays
Black walls reduce side scatter by 93%, maintaining linearity in 30-minute fluorescence kinetics. -
Evaporation Control for Micro-Volume Reactions
Black lid limits volume loss to <2μl over 45 minutes at 37°C incubation. -
Consistent Optical Path for Reproducibility
±0.1mm pathlength tolerance ensures <5% variation in Vmax across replicates.
TOQUARTZ® solution
A pharmaceutical R&D team conducting enzyme kinetics with 50μl fluorescent substrate experienced 20% Vmax deviation due to evaporation and light scatter. Switching to TOQUARTZ® cuvettes reduced deviation to 4.6%, stabilized kinetic curves, and cut reagent usage by 60% per assay.
TOQUARTZ® Customization Options for Quartz Micro Fluorescence Cuvette with Black Walls and Lid
Custom Dimensions
- Non-standard pathlengths
- Custom internal volumes
- Special window sizes and shapes
- Instrument-specific external dimensions
Specialized Features
- Flow-through ports and connectors
- Temperature control jackets
- Screw cap or septum-compatible designs
- Multi-cell arrays for high-throughput
Custom Design Process
- Consultation: Initial discussion to understand your application requirements, technical specifications, and performance expectations. Our engineers work closely with you to define the optimal solution.
- Design & Prototyping: Our technical team develops detailed specifications and produces initial prototypes for your evaluation. We can provide 3D renderings or physical samples based on project requirements.
- Testing & Refinement: Prototypes undergo rigorous optical and dimensional testing. We work with you to refine the design based on real-world performance evaluation and feedback.
- Production & Quality Control: Once the design is finalized, we move to production with comprehensive quality control at every stage. Each custom cuvette is individually inspected to ensure it meets your specifications.
Usage Guide of Quartz Micro Fluorescence Cuvette with Black Walls and Lid
Handling Recommendations
- Always handle cuvettes by the frosted or non-optical sides to avoid fingerprints
- Place on clean, soft surfaces to prevent scratches on optical windows
- When filling micro-volume cuvettes, use precision pipettes to avoid air bubbles
- Ensure proper orientation in the instrument (light path aligned with optical windows)
- Secure the lid properly to prevent evaporation during measurement
Cleaning Procedures
- Rinse immediately after use with appropriate solvent (typically deionized water)
- For stubborn residues, soak in 5% laboratory detergent solution
- Rinse thoroughly with deionized water and spectroscopic grade solvent
- Allow to air dry in a dust-free environment or use filtered compressed air
- Never use abrasive materials or brushes on optical surfaces
Storage Guidelines
- Store in original packaging or dedicated cuvette holders
- Keep in a clean, dry environment away from dust
- Avoid temperature extremes and rapid temperature changes
- Inspect for cleanliness before storage to prevent residue buildup
- For long-term storage, consider a desiccator to prevent humidity damage
Chemical Compatibility
- Compatible with most laboratory solvents, acids, and bases
- Avoid hydrofluoric acid (HF) and hot concentrated phosphoric acid
- The black coating may be affected by certain organic solvents
- For unusual solvents, test compatibility on a spare cuvette first
- Temperature limit for black coating is approximately 200°C
Need Technical Assistance with Quartz Micro Fluorescence Cuvettes with PTFE Lid?
Contact us today to discuss your project.
Why Partner with TOQUARTZ
Direct Factory Advantage
As a direct manufacturer, we can cut out the numerous intermediate links.
Engineering Expertise
Technical team guides clients from material selection to design optimization, translating specs into deliverables.
Flexible Manufacturing
Handling standard & custom orders via small-batch expertise and prototyping rigor to meet urgent deadlines.
Quality
Assurance
Pre-shipment 3-step validation:
1. dimensional accuracy,
2. material purity ,
3. performance thresholds
Global Supply Chain
Reliable global logistics to industrial hubs (DE/US/JP/KR priority) with trackable milestones.
Releted Products
As a specialized manufacturer with direct factory capabilities, TOQUARTZ provides both standard and custom quartz solutions with engineering support throughout the specification and implementation process.
FAQ
Q: Is the Quartz Micro Fluorescence Cuvette with Black Walls and Lid compatible with standard spectrophotometer holders?
A: Yes, it follows the standard 12.5mm × 12.5mm × 45mm external dimensions, compatible with most commercial fluorescence and UV-VIS instruments.
Q: Are Quartz Micro CHow does the Quartz Micro Fluorescence Cuvette with Black Walls and Lid improve enzyme kinetics assays?
A: It provides stable optical conditions and prevents evaporation, allowing accurate time-resolved fluorescence readings in micro-volume enzyme reactions.
Q: Can the Quartz Micro Fluorescence Cuvette with Black Walls and Lid be used in thermal cycling or heated fluorescence assays?
A: Yes, it is suitable for thermal assays up to 200°C, with the black lid preventing evaporation and maintaining optical stability during temperature changes.
Q: Is the lid of the Quartz Micro Fluorescence Cuvette with Black Walls and Lid airtight or evaporation-proof?
A: The lid is designed to minimize evaporation during standard fluorescence assays, reducing volume loss to less than 2μl over 45 minutes at 37°C.
Q: How does the black lid of the Quartz Micro Fluorescence Cuvette with Black Walls and Lid help in fluorescence measurements?
A: The black lid prevents ambient light from entering the cuvette and minimizes sample evaporation, ensuring consistent optical conditions during long-duration or thermal assays.
Contact our engineering team for technical consultation and pricing. We’ll help you select the optimal specifications for your application requirements.