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Evaporation-Controlled Quartz Micro Fluorescence Cuvette with PTFE Lid for Spectroscopy -TOQUARTZ®
Features of Quartz Micro Fluorescence Cuvette with PTFE Lid
TOQUARTZ® Quartz Micro Fluorescence Cuvettes with PTFE Lids are manufactured to meet the highest industry standards for laboratory spectroscopy applications. These precision-engineered cuvettes deliver exceptional performance across a wide range of analytical procedures.
Design Features
- PTFE lid provides secure sealing to prevent sample evaporation
- Micro-volume capacity for precious sample conservation
- Standard 12.5 x 12.5mm cross-section for compatibility with most instruments
- Uniform wall thickness for consistent optical performance
Optical Performance
- UV-VIS-NIR transmission range (200-2500nm)
- No absorption peaks throughout the spectrum range
- Precise optical path length (±0.1mm tolerance)
- Polished surfaces with no bubbles or scratches
Material Properties
- High-purity quartz (≥99.98% SiO₂, Max. up to 99.995%) for optimal optical performanc
- Superior chemical resistance against acids, bases, and corrosive agents
- Thermal stability up to 600°C (model dependent)
- Low thermal expansion coefficient: 5.5×10⁻⁷ /°C
- Resistant to acids and alkalis(except HP)
- Temperature resistance up to 1100°C
- Custom dimensions available
Technical Specifications & Dimensions of Quartz Micro Fluorescence Cuvette with PTFE Lid
Technical Specifications
Physical Properties
| Parameter | Specification |
| Material | High-purity quartz |
| Purity | ≥99.98% SiO2, Max. up to 99.995% |
| Density | 2.2 g/cm³ |
| Thermal Expansion | 5.5×10-7/°C |
| Hardness | 5.5-6.5 Mohs |
Chemical Properties
| Parameter | Specification |
| Acid Resistance | Excellent |
| Alkali Resistance | Excellent |
| Solvent Resistance | Excellent |
| Water Absorption | Negligible |
| Chemical Inertness | Very High |
Optical Properties
| Parameter | Specification |
| Transmission Range | 200-2500nm |
| Refractive Index | 1.46 (at 589.3nm) |
| UV Cutoff | 170nm |
| IR Cutoff | 3500nm |
Size Chart
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4001 | 350μl Quartz Micro Fluorescence Cuvette with PTFE Lid | 200nm-2500nm | 10mm | 350μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4014 | 560μl Quartz Micro Fluorescence Cuvette with PTFE Lid | 200nm-2500nm | 4mm | 560μl | 600℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4002 | 700μl Quartz Micro Fluorescence Cuvette with PTFE Lid | 200nm-2500nm | 10mm | 700μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4009 | 700μl Quartz Micro Fluorescence Cuvettes with PTFE Lid | 200nm-2500nm | 10mm | 700μl | 600℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4029 | 1050μl Quartz Micro Fluorescence Cuvette with PTFE Lid | 200nm-2500nm | 10mm | 1050μl | 600℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4026 | 1400μl Quartz Micro Fluorescence Cuvette with PTFE Lid | 200nm-2500nm | 10mm | 1400μl | 100℃ | 12.5×12.5x45mm |
| Model | Description | Wavelength | Path Length |
Volume | Thermal Stability | Outline Dimension |
| AT-BSM-4035 | 1750μl Quartz Micro Fluorescence Cuvette with PTFE Lid | 200nm-2500nm | 10mm | 1750μl | 100℃ | 12.5×12.5x45mm |
TOQUARTZ® Solving Research Challenges with Quartz Micro Fluorescence Cuvettes with PTFE Lid
Quartz Micro Fluorescence Cuvette with PTFE Lid in qPCR-Based DNA Quantification
Key Advantages
-
Evaporation barrier during thermal cycling
PTFE lid reduced sample volume loss by 92% during 40-cycle qPCR at 95°C. -
Maintains DNA concentration consistency
Fluorescence signal deviation stayed within ±1.2% across replicates using sealed cuvettes. -
Enables accurate quantification in <500μl volumes
Supports micro-volume assays with as little as 350μl without concentration drift.
TOQUARTZ® solution
A molecular diagnostics lab in Boston reported inconsistent Ct values due to evaporation in open cuvettes during qPCR. After switching to TOQUARTZ® cuvettes with PTFE lids, their DNA quantification variance dropped from ±3.8% to ±0.9%, and sample loss was reduced by over 90%, enabling reliable results even with 400μl input volumes.
Quartz Micro Fluorescence Cuvette with PTFE Lid in Fluorescent Protein Assays in Biotech R&D
Key Advantages
-
Reduces oxygen-induced fluorescence quenching
PTFE lid lowered quenching rate by 78% in GFP assays over 30 minutes. -
Minimizes protein oxidation during exposure
Fluorescence intensity retained 96% after 1-hour exposure under excitation light. -
Prevents contamination from airborne particles
Sealed design reduced contamination-related assay failures by 85% in open-bench setups.
TOQUARTZ® solution
A biotech startup in Berlin working on fluorescent protein variants faced 25–30% signal loss due to oxidation in open cuvettes. After adopting TOQUARTZ® sealed cuvettes, they maintained >95% signal integrity over 60 minutes and reduced assay repeat rates by 70%, significantly improving throughput and data reliability.
Quartz Micro Fluorescence Cuvette with PTFE Lid in Trace Pollutant Detection in Water Testing Labs
Key Advantages
-
Prevents solvent evaporation during fluorescence scans
PTFE lid retained >98% of methanol volume over 20-minute analysis at 25°C. -
Maintains analyte concentration for low-ppb detection
Enabled consistent detection of benzo[a]pyrene at 1.2 ppb with <±1.5% variance. -
Shields samples from airborne particulate contamination
Reduced false positives from airborne interference by 60% in open-lab environments.
TOQUARTZ® solution
An environmental testing lab in Warsaw struggled with inconsistent PAH readings due to solvent evaporation in open cuvettes. Switching to TOQUARTZ® cuvettes with PTFE lids stabilized their benzo[a]pyrene detection at 1.2 ppb with <±1.5% variance, down from ±8%, and eliminated the need for sample re-runs in 90% of cases.
TOQUARTZ® Customization Options for Quartz Micro Fluorescence Cuvette with PTFE Lid
Dimensional Customization
TOQUARTZ® engineering team can produce cuvettes with custom dimensions to fit proprietary instruments or specialized applications with tolerances down to ±0.1mm.
- Custom optical path lengths
- Specialized internal volume
- Non-standard external dimensions
Specialized Surface Treatments
Surface treatments can significantly improve signal-to-noise ratios in sensitive fluorescence applications, enhancing detection limits by up to 40%.
- Black wall coatings for low background
- Anti-reflective coatings for specific wavelengths
- Frosted surfaces for reduced stray light
Specialized Lid Options
Specialized lid options can address specific needs such as working with volatile samples, extended high-temperature applications, or kinetic studies requiring reagent addition.
- Screw-top lids for enhanced sealing
- High-temperature lids (up to 600°C)
- Septum ports for sample addition
Usage Guide of Quartz Micro Fluorescence Cuvette with PTFE Lid
Handling and Storage
- Always handle cuvettes by the frosted or non-optical surfaces to prevent fingerprints on optical windows.
- Store in the original protective case or in a dust-free environment.
- Ensure the PTFE lid is securely seated but not overtightened to prevent stress on the quartz material.
- Store away from harsh chemicals that might attack the PTFE lid material (strong oxidizers).
- Protect from mechanical shock; though durable, quartz can chip or crack if dropped.
Cleaning Procedures
- Rinse immediately after use with appropriate solvent (typically deionized water, followed by ethanol or acetone).
- For protein residues, use enzymatic cleaners designed for laboratory glassware.
- For stubborn contaminants, soak in 2-5% Hellmanex™ solution for 12 hours (do not soak PTFE lids).
- Rinse thoroughly with ultrapure water and allow to air dry in a dust-free environment.
- Never use abrasive materials or brushes that might scratch the optical surfaces.
Sample Loading
- Fill to the recommended volume level, ensuring the optical path is completely filled but avoiding overflow.
- Use micropipettes with appropriate tips to avoid scratching the inner walls.
- Check for air bubbles before measurement and tap gently to release if present.
- For temperature-sensitive measurements, pre-equilibrate the cuvette at the target temperature.
Measurement Best Practices
- Orient the cuvette with the optical windows facing the light path (typically the clear sides).
- For fluorescence, typically measure at a 90° angle to the excitation beam for best results.
- Allow temperature equilibration for at least 2 minutes before measurement if temperature control is critical.
- Use the same cuvette for reference and sample measurements when possible for highest precision.
- For kinetic measurements, ensure the PTFE lid is properly seated to prevent evaporation over time.
Need Technical Assistance with Quartz Micro Fluorescence Cuvettes with PTFE Lid?
Contact us today to discuss your project.
Why Partner with TOQUARTZ
Direct Factory Advantage
As a direct manufacturer, we can cut out the numerous intermediate links.
Engineering Expertise
Technical team guides clients from material selection to design optimization, translating specs into deliverables.
Flexible Manufacturing
Handling standard & custom orders via small-batch expertise and prototyping rigor to meet urgent deadlines.
Quality
Assurance
Pre-shipment 3-step validation:
1. dimensional accuracy,
2. material purity ,
3. performance thresholds
Global Supply Chain
Reliable global logistics to industrial hubs (DE/US/JP/KR priority) with trackable milestones.
Releted Products
As a specialized manufacturer with direct factory capabilities, TOQUARTZ provides both standard and custom quartz solutions with engineering support throughout the specification and implementation process.
FAQ
Q: How do Quartz Micro Cuvettes with PTFE Lid prevent evaporation during fluorescence analysis?
A: The PTFE lid forms a tight seal that significantly reduces sample evaporation, especially during prolonged fluorescence measurements or thermal cycling.
Q: Are Quartz Micro Cuvettes with PTFE lid compatible with standard fluorescence spectrophotometers?
A: Most TOQUARTZ® Quartz Micro Cuvettes with PTFE lid have a standard 12.5×12.5mm outer dimension, making them compatible with common fluorescence instruments.
Q: What is the optical path length tolerance in Quartz Micro Cuvettes with PTFE lid?
A: TOQUARTZ® Quartz Micro Cuvettes with PTFE lid maintain an optical path length tolerance within ±0.1mm, ensuring consistent fluorescence signal across replicates.
Q: Are Quartz Micro Cuvettes with PTFE lid suitable for volatile solvent-based fluorescence assays?
A: Absolutely. The PTFE lid design helps retain volatile solvents like methanol or acetonitrile, maintaining analyte concentration during trace-level fluorescence detection.
Q: How do Quartz Micro Cuvettes with PTFE lid perform in low-ppb fluorescence pollutant detection?
A: Quartz Micro Cuvettes with PTFE Lid provide high optical clarity and solvent retention, enabling stable detection of pollutants like PAHs at concentrations as low as 1.2 ppb.
Contact our engineering team for technical consultation and pricing. We’ll help you select the optimal specifications for your application requirements.