2025 Leitfaden: Quarz, Glas oder Kunststoff? So wählen Sie eine 96-Well-Platte mit 80% UV-Durchlässigkeit

Selecting the right 96-well plate is crucial for accurate UV-based assays and high-throughput laboratory workflows.

Quartz 96-well plates deliver >80% UV transmission at 220nm, outperforming glass and plastic for nucleic acid quantification, protein crystallization, and advanced photometric applications.

This guide provides a data-driven comparison, selection matrix, and supplier evaluation framework for engineers and scientists in 2025.

Defining the 96-Well Plate and Its Critical Role in UV Applications

A 96-well plate is a standardized microplate used for parallel sample analysis in laboratories. Quartz 96-well plates are engineered from high-purity fused silica, offering exceptional UV transparency and chemical resistance.

Standard Specifications and Common Uses in Laboratory Settings

Standard 96-well plates have wells arranged in an 8×12 grid, with volumes ranging from 100μL to 400μL per well. They are used in ELISA¹, PCR², and spectrophotometric assays.

Quartz plates are chosen for UV-based protocols, while glass and plastic are used for visible-light or disposable applications. Engineers should match plate material to assay requirements.

Why Quartz Excels in UV Transparency for Sensitive Assays

Quartz transmits >80% of UV light at 220nm, compared to 10–30% for glass and <5% for plastic. This high transmission is essential for DNA/RNA quantification and protein analysis.

Quartz also resists thermal and chemical degradation, ensuring consistent results in repeated use. Its low background absorption minimizes interference in sensitive measurements.

Essential Properties: Thermal, Chemical, and Optical Performance of Quartz, Glass, and Plastic

Material properties directly impact assay sensitivity, durability, and compatibility with UV-based protocols.

UV Transmission Rates: Comparing Quartz (80% at 220nm), Glass, and Plastic

Quartz plates maintain high UV transmission across the 190–400nm range. Glass and plastic show rapid transmission loss below 300nm, limiting their use in UV assays.

Always request batch-specific transmission data and compare to assay requirements. Suppliers should provide certified UV-Vis-NIR curves.

Durability Under High-Temperature and Chemical Exposure

Quartz plates withstand repeated autoclaving and exposure to acids, bases, and solvents. Glass may crack under thermal cycling, and plastic can deform or leach contaminants.

Engineers should specify plate material based on expected temperature and chemical exposure in their workflow.

Selecting Materials Based on Specific Application Requirements

Choose quartz for UV-based, high-sensitivity, or high-temperature assays. Glass is suitable for visible-light protocols and moderate chemical exposure. Plastic is best for disposable, low-cost, or non-UV applications.

Technical Comparison: Quartz vs. Glass vs. Plastic for Key Applications

Comparing materials helps engineers and buyers make informed decisions for UV-based assays and high-throughput screening.

Performance in Nucleic Acid Quantification: Data on Background Absorption

Quartz plates minimize background absorption, supporting accurate DNA/RNA quantification at 220–260nm. Glass and plastic introduce higher background, reducing sensitivity and accuracy.

For critical assays, always select quartz and request data on background absorbance and signal-to-noise ratio.

Suitability for Protein Crystallization: Thermal Stability Analysis

Kristallisation von Proteinen³ requires plates that resist thermal cycling and chemical attack. Quartz plates maintain dimensional stability and clarity after repeated autoclaving.

Glass may crack or cloud, while plastic can deform or absorb solvents. Quartz is the preferred choice for reproducible, high-quality crystal growth.

Decision Framework: Choosing the Optimal Material for Your Experimental Needs

A structured decision framework helps balance performance, cost, and operational requirements.

Cost-Benefit Analysis: Balancing Performance and Budget

Quartz plates have a higher upfront cost but offer superior durability and reusability. Glass is moderately priced but less durable, while plastic is cheapest but disposable.

Consider total cost of ownership, including replacement frequency, cleaning, and assay accuracy. For high-value assays, quartz often delivers the best ROI.

Scenarios Where Glass or Plastic Outperform Quartz

Glass may be preferable for visible-light assays or when moderate chemical resistance is sufficient. Plastic is ideal for high-throughput screening where cost and disposability are priorities.

Engineers should match material to assay needs, not just price.

Supplier Evaluation: Key Certifications for Quartz 96-Well Plates

Supplier selection is critical for ensuring consistent quality and regulatory compliance.

ISO 8037 Standards and Quality Verification Tests

Look for ISO 8037 and ISO 9001 certification. Suppliers should provide batch-specific UV transmission and OH content data, as well as surface finish inspection (e.g., scratch-dig 10-5).

Request test reports and audit supplier quality systems for critical applications.

Assessing Technical Capabilities of Leading Manufacturers

Evaluate suppliers’ ability to provide custom sizes, surface finishes, and grade certifications. Reliable suppliers offer engineering consultation, rapid prototyping, and post-sale support.

Traceability and documentation are essential for regulated environments.

Cost and Timeline Considerations in Custom Quartz Plate Procurement

Cost drivers include material grade, thickness, surface finish, and order quantity. Tighter tolerances and higher purity grades increase price.

Lead times for standard plates are typically 2–4 weeks; custom orders may require 4–6 weeks. Bulk orders and long-term contracts can reduce unit costs.

Request detailed quotes and confirm all specifications before ordering. Plan ahead for urgent projects and communicate deadlines clearly.

Factors Influencing Pricing: Material Purity and Customization

Higher purity and custom dimensions increase cost. Standard sizes and grades are more economical and readily available.

Negotiate MOQs and delivery terms to optimize budget and timeline.

Typical Lead Times and Strategies for Minimizing Delays

Early engagement with suppliers and clear communication of requirements help minimize delays. Request expedited production or shipping for urgent needs.

Troubleshooting Common Issues: Cleaning, Autoclaving, and Haze Prevention

Proper maintenance extends the life and performance of quartz plates.

Best Practices for Maintaining Optical Clarity Post-Use

Clean quartz plates with acid baths or ultrasonic cleaning. Rinse with deionized water and dry in a dust-free environment.

Avoid abrasive cleaners and HF unless specified. Inspect for microcracks and haze after each use.

Validating Performance After High-Temperature Exposure

After autoclaving or high-temperature use, verify UV transmission and surface clarity. Replace plates showing signs of devitrification or clouding.

Document maintenance and inspection results for traceability.

Implementation and Validation: Ensuring Optimal Use of Quartz 96-Well Plates

Proper implementation and validation ensure reliable results and long-term value.

Step-by-Step Guide for Initial Setup and Calibration

• Inspect plates for defects and verify batch certificates.
• Calibrate spectrophotometers using reference standards.
• Document initial transmission and background absorbance.

Methods for Monitoring Performance and Detecting Degradation

Schedule regular performance checks and cleaning. Monitor for changes in transmission, clarity, or physical integrity.

Replace plates as needed to maintain assay accuracy and compliance.

FAQ (Häufig gestellte Fragen)

What is the typical UV transmission rate for a quartz 96-well plate?
High-purity quartz plates transmit 80–92% at 220nm and >90% at 254nm, far exceeding glass or plastic.

How do I clean and reuse quartz 96-well plates?
Verwenden Sie Säurebäder oder Ultraschallreinigung, spülen Sie mit entionisiertem Wasser und prüfen Sie auf Risse. Vermeiden Sie Scheuermittel und HF, sofern nicht anders angegeben.

Welche Zertifizierungen sollte ich von einem Quarzplattenlieferanten verlangen?
Look for ISO 9001, ISO 8037, and batch-specific UV transmission and OH content data.

Wie schneidet Quarz im Vergleich zu Glas und Kunststoff bei UV-Tests ab?
Quarz bietet eine höhere UV-Durchlässigkeit, eine geringere Hintergrundabsorption und eine längere Lebensdauer, was es für anspruchsvolle UV-Anwendungen unentbehrlich macht.

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